Composite

Part:BBa_K3009039:Design

Designed by: Alisa Zinina   Group: iGEM19_Freiburg   (2019-10-21)


This is a trimolecular fusion of tRNA Synthetase and SDP5, MCP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1138
    Illegal AgeI site found at 1507
    Illegal AgeI site found at 1606
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 304


Design Notes

SPD5 has to be fused to MCP and not the Synthetase, the C terminus has to be free


Source

C.elegans, MS2 bacteriophage, M.jannashii

References