Composite
Part:BBa_K3009039:Design
Designed by: Alisa Zinina Group: iGEM19_Freiburg (2019-10-21)
This is a trimolecular fusion of tRNA Synthetase and SDP5, MCP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1138
Illegal AgeI site found at 1507
Illegal AgeI site found at 1606 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 304
Design Notes
SPD5 has to be fused to MCP and not the Synthetase, the C terminus has to be free
Source
C.elegans, MS2 bacteriophage, M.jannashii